Syllabus for General Biology Lab
(Subject code: CB3202)
Spring semester, 2016
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Department
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Subject
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Credits
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Class Hours/Wk
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Attendee
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Chem & Bio
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General Biology Lab
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1
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2
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1. Subject outline
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Aims and objectives
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■ Learn how to write a laboratory notebook
■ Learn basic lab techniques focusing on the DNA biology
■ Learn the proper usage of various lab equipments
■ Learn how to interpret and analyze results of experiments
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Key concepts
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● Laboratory apparatus and terminology
● Laboratory safety
● How to find research papers
● Components of research papers
● Preparation of medium and sterilization
● Micropipetting, centrifugation, spectrophotometer
● Isolation of genomic DNA
● Amplification of DNA
● DNA sequence analysis
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Suggestion
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Read the protocols before coming into lab
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2. Textbook and reference book
◆ References (available in KSA library)
i) Micklos DA et al. (2003) DNA Science-a first course (2nd edition)
ii) Reed R et al. (2007) Practical Skills in Biomolecular Sciences (3rd edition)
iii) Ratledge and Kristiansen (2001) Basic Biotechnology (2nd edition)
3. Assessment method
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Lab Work1
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Group Presentation 2
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Lab Notebooks 3
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Pre-lab, Quiz4
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Attendance5
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Total
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40
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15
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20
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20
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5
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100
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1: This component of your grade is based on your performance, cooperativity, clean-up after work, and attitude. Good performance and experimental result will gain a point. 매주 자신들의 실험활동 중 주요장면을 사진 또는 동영상 촬영해서 보관하세요. 마지막 발표 때에 이 자료를 이용하여 정리하는 데에 사용합니다.
2: The purpose of the group presentation is to ensure that 1) you understand the whole project of general biology lab 1 and 2) you have chance to further study about related lab methods.
3: You will keep a personal notebook for lab protocols, results, and analysis of the results. Keep a good record.
4: Before you come to the lab you read the manual book and make your own flowchart showing the schematic view of the lab work. (Write them on a separate A4 sheet and submit every week) And worksheets (quiz) will be given to check your understanding of experiments. Before coming to the lab, study the protocol in preparation of the quiz and lab procedure itself as well.
5: Attendance in the lab is mandatory. An absence will cost 2 points. If you come late it will cost 0.5 points. Be on time.
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Week
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Topics
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Class activity
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Week 1
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1. Introduction to the course
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Week 2
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2. Laboratory safety
3. Guidelines for a lab notebook
4. Laboratory equipment and terminology
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Week 3
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5. Preparation of LB medium, Sterilization
6. Sampling and Inoculation
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Week 4
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7. Preparation of a Streak Plate for Single Colonies
8. Use of Micropipette and Centrifuge
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Week 5
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9. Isolation of Genomic DNA from Bacteria
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Week 6
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10. Measuring DNA Concentration by Spectrophotometer
11. PCR of 16S rDNA
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Week 8
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12. DNA electrophoresis
13. PCR product purification
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Week 8
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Mid-term exam(4/11~4/15)
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Week 9
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14. DNA Ligation
15. E. coli Transformation
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Worksheet
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Week 10
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16. Purification of Bacterial Plasmid
17. Restriction enzyme digestion
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Week 11
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18. Agarose gel electrophoresis of restricted plasmids
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Week 12
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19. Sanger's DNA sequencing method (theory)
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Theory, Worksheet
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Week 13
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20. DNA analysis using BLAST (Bring your notebook.)
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Summary Poster
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Week 14
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SAF(~5/24)
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Week 15
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21. Group presentation
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Week 16
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현충일
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Week 17
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Final-term exam(6/16~6/22)
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4. Weekly schedule of class (Mon class)
